Search results for "Sperm washing"

showing 3 items of 3 documents

First report of the absence of viral load in testicular sperm samples obtained from men with hepatitis C and HIV after washing and their subsequent u…

2008

Human immunodeficiency virus and hepatitis C infections are sexually transmitted diseases that require sperm samples to be pretreated to eliminate the viral presence before their safe use in assisted reproduction treatments. In this report we describe our experience with sperm washing protocols applied to sperm cells from testicular biopsies as well as the results obtained in subsequent assisted reproduction treatments on seropositive males that are also azoospermic.

AdultMaleendocrine systemSperm RetrievalReproductive Techniques AssistedBiopsySexually Transmitted DiseasesSemenHIV InfectionsHepacivirusTesticleAndrologyTestismedicineHumansSperm Injections IntracytoplasmicAzoospermiaurogenital systembusiness.industrySperm washingObstetrics and GynecologyHIVHepatitis CMiddle AgedViral Loadmedicine.diseaseSpermVirologyHepatitis CSpermatozoamedicine.anatomical_structureReproductive MedicineSperm RetrievalViral diseasebusinessViral loadFertility and sterility
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Effect of sperm concentration and storage temperature on goat spermatozoa during liquid storage

2020

The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of …

L53 Animal physiology - Reproductionmedicine.medical_treatmentDNA fragmentationmitochondrial activitylaw.invention0302 clinical medicineL10 Animal genetics and breedinglawlcsh:QH301-705.5Semen qualitySperm motilityreproductive and urinary physiologyLiquid Storageliquid storage030219 obstetrics & reproductive medicineMurciano-GranadinaExtenderSperm washingMotilityMitochondrial Activity04 agricultural and veterinary sciencesBiología y Biomedicina / BiologíamotilityDNA fragmentationGeneral Agricultural and Biological SciencesGenetic improvementendocrine systemMotilityShelf lifeSemen ExtendersSemenDNA FragmentationBiologyGeneral Biochemistry Genetics and Molecular BiologyAi (artificial insemination)ArticleAndrology03 medical and health sciencesSemenmedicineU10 Mathematical and statistical methodsgoat spermGeneral Immunology and Microbiologyurogenital systemArtificial insemination0402 animal and dairy scienceGoat Sperm040201 dairy & animal scienceSpermFrozen storagelcsh:Biology (General)
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Processing Sperm Samples in HIV-Positive Patients

2013

Human immunodeficiency virus is an important public health problem; about 40 million people around the world are actually infected with HIV type 1 virus. Furthermore, prolonged survival of patients under highly active antiretroviral therapy has given rise to a new demand from SDC couples who wish to have children. HIV serodiscordant couples with the male infected wish to make use of assisted reproduction techniques in combination with semen washing in order to decrease the risk of transmission to the partner or to the newborn. Thus it is essential to test the effectiveness of techniques aimed at reducing HIV and HCV loads in infected semen using molecular biology tests. Nevertheless, its ef…

medicine.medical_specialtyTransmission (medicine)business.industryPublic healthImmunologySerodiscordantSperm washingMedicineSemenbusinessViral loadSpermVirus
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